Plants of the Cerrado with antimicrobial effects against Staphylococcus spp. and Escherichia coli from cattle
نویسندگان
چکیده
BACKGROUND Both diarrhea in calves and mastitis in cows limit cattle production. The bacteria involved in these diseases have shown multi-resistance to antimicrobials, however plant metabolites therefore can provide an alternative method of control. This study selected and characterized Cerrado plant extracts showing inhibitory effects against Escherichia coli and Staphylococcus spp. from cattle. Thirteen leaf extracts were initially screened and diameters of inhibition zones produced against the pathogens were recorded using an agar disk diffusion method. Total condensed tannin contents were determined and antibacterial activities were analyzed after tannin removal from the five selected extracts. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were evaluated by macro-dilution antimicrobial susceptibility tests, and the extracts were characterized by high performance liquid chromatography. RESULTS Inter- and intra-specific bacterial variations in the susceptibility to the extracts were detected. The aqueous extract (AE) from Caryocar brasiliense Cambess. leaves produced larger inhibition zones against E. coli strains than did other selected extracts. However, the AE from Schinopsis brasiliensis was the most effective against Staphylococcus spp. strains (P < 0.001). The MIC of ethanolic extracts (EE) from C. brasiliense (0.27 mg/mL) and S. brasiliensis (0.17 mg/mL) were lower than those of other extracts. The MIC and MBC of the Annona crassiflora EE were 6.24 mg/mL for all bacteria. Flavonoids were the main metabolites detected in the A. crassiflora EE as well as in the AE and EE from C. brasiliense, while tannins were the main metabolites in the S. brasiliensis leaf extracts. CONCLUSION The AE from C. brasiliense was more effective against Gram-negative bacteria, while the AE from S. brasiliensis was more effective against Gram-positive bacteria. A. crassiflora EE and S. brasiliensis extracts are potent bactericide. After removal of the tannins, no antimicrobial effects were observed, indicating that these metabolites are the main active antibacterial components.
منابع مشابه
Evaluating Antimicrobial Effects of Centaurea Plant’s Essential Oil on Pathogenic Bacteria: Staphylococcus Aureus, Staphylococcus Epidermidis, and Escherichia Coli Isolated from Clinical Specimens
Background & Objectives :Nowadays, development of drug resistance against chemical antimicrobial drugs has attracted attention using medicinal plants in treatment of infections. The aim of this study is to evaluate the antimicrobial effects of two species of Centaurea plant’s essential oil on drug resistant clinical isolates of three pathogenic isolates. Materials & Methods :T...
متن کاملInvestigating Antibacterial Effect of Thyme (Thymus persicus) and Pennyroyal (Mentha longifolia) Alcoholic and Aqueous Extracts against Isolated Bacteria from Domestic Cheeses
Background & Objective: Food poisonings caused by consumption of domestic dairy products such as cheese due to Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) are one of the hygienic problems in foodstuffs. Nowadays, using natural preservatives instead of synthetic preservatives in foods has been regarded increasingly and among these natural compounds, medicinal plants comprise t...
متن کاملAntimicrobial Effects of Grape and Pomegranate Waste Extracts against two Foodborne Pathogens
In recent years, increasing in the level of public health consciousness has led to a drastic decrease in the use of synthetic food preservatives. Therefore, there has been a growing demand for novel antimicrobials. Apart from that, finding low cost sources has been always an important issue for food marketers. The present study was designed to investigate in-vitro antibacterial activities of gr...
متن کاملChemical Composition, Antioxidant Activity and Antimicrobial Effect of Rosa damascena Mill. Essential Oil Against Staphylococcus aureus, Escherichia coli and Salmonella typhi
Food preservation is an action or a method of maintaining foods at a desired level of properties or nature for their maximum benefits. In general, each step of handling, processing, storage, and distribution affects the characteristics of food, which may be desirable or undesirable. Thus, understanding the effects of each preservation method and handling procedure on foods is critical in food p...
متن کاملAntagonistic Effects of Lactobacilli spp. against Ciprofloxacin-Resistant Uropathogenic Escherichia coli Strains
Background and Objectives: Recently, the use of probiotics for the treatment of Urinary Tract Infections has become more popular. The use of probiotics in therapy is useful as only a few side effects such as destruction of resistant bacteria or disturbance of the intestinal microbiota have been reported. The aim of this study was to evaluate the probiotic effects of lactic acid bacteria by co-a...
متن کامل